Fig. 5.

ASPP2 inhibits lipid metabolism through the PPARγ-mTOR signaling pathway in alcohol-induced hepatocyte injury. (A and B) Western blot (A) and quantification analyses (B) of ASPP2, PPARγ, phospho-mTOR, phospho-S6, phospho-p70S6K, and β-actin in primary hepatocytes (Control and ASPP2 siRNA) with the indicated treatment. (C, D, E, and F) The mRNA levels of genes involved in lipid metabolism in primary hepatocytes (Control and ASPP2 siRNA) with the indicated treatment measured by real-time PCR. (G) ALT and AST levels in primary hepatocytes cell supernatant (Control and ASPP2 siRNA) with the indicated treatment. (H) TC and TG levels in primary hepatocytes cell supernatant (Control and ASPP2 siRNA) with the indicated treatment. nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. The values represent the means ± SEMs (n = 6 in each group). Independent-samples T tests between two groups were used for statistical analysis, and one-way ANOVA followed by Bonferroni post hoc tests for multiple comparisons were used for statistical analyses