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. 1982 Aug 1;205(2):313–320. doi: 10.1042/bj2050313

Purification and radioimmunoassay of rat lactate dehydrogenase A and B subunits.

T J Beebee, D S Carty
PMCID: PMC1158483  PMID: 7138505

Abstract

We have developed procedures for purifying lactate dehydrogenase isoenzymes from rat tissues that involve two affinity-chromatography steps and that facilitate the isolation of milligram quantities of highly purified proteins within 2--3 days. Antibodies raised against pure A and B subunits in rabbits and hens were used in radioimmunoassays and showed negligible cross-reactivity with heterologous subunits. The radioimmunoassays provide a sensitive method for measuring nanogram amounts of A-subunit and B-subunit polypeptides in tissue homogenates and were employed to characterize the enzyme purification procedures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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