Fig. 2.

Intercellular connections between LCMV-GP-1-FLAG infected cells contain both F-actin and tubulin, as well as virion component GP-1. A549 cells were infected with rLCMV-GP1-FLAG at an MOI of 0.2. At 24 hpi, cells were fixed and then stained with DAPI and phalloidin (actin) alongside antisera specific for GP-1 (cyan) and a β-tubulin-specific Affimer (green), and then visualized by confocal microscopy. A magnified region of interest (white box in the central row) is shown on the middle row, zoom-1, where multiple cell-cell connections are visualized by staining with both tubulin and F-actin, shown further magnified in the bottom row, zoom-2. Additional points of interest are indicated by arrows to highlight punctate regions of LCMV GP-1 present in uninfected neighbouring cells, extending beyond F-actin and β-tubulin staining.