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. 2024 Nov 22;14:28961. doi: 10.1038/s41598-024-79397-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Stimulated emission depletion microscopy reveals the structural organisation of cell-to-cell connections between A549 cells infected with rLCMV-GP1-FLAG. (A) A549 cells were infected with rLCMV-GP1-FLAG at an MOI of 0.2. and at 24 hpi, cells were fixed, stained for GP-1-FLAG (cyan) and F-actin (red), then visualized by stimulated emission depletion microscopy (STED). An infected cell (left) and a zoom (right) region of interest (white box) are shown. Points of interest are indicated (arrows) to highlight punctate regions of LCMV GP-1 that exist along an F-actin branch. The bottom right panel is a magnified version of the region within the white box from the bottom left panel. (B) Line scan analysis of the region of interest in the zoomed merged image (white box), showing GP-1 peak intensities do not closely correspond to those of F-actin at this resolution.