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. 2024 Nov 23;30:227. doi: 10.1186/s10020-024-00990-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Fibroblasts induce cystic organoid growth. (a) Scheme of the experimental layout. (b) Representative phase contrast images of organoids cultured for 21 days (noFB: AT2 cells cultured without fibroblasts (FB); FB: AT2 cells cultured in the presence of fibroblasts, P1: passage 1). (c) Quantification of the morphology of the alveolar organoids. Data were compared by unpaired t-test (***p < 0.001). Each data point represents an independent experiment. (d) Quantification of the diameter of the alveolar organoids. Pooled results from 3 independent experiments. Data were compared by Mann-Whitney test (****p < 0.0001)