Fig. 2.

Silencing TaZFP8 increases wheat resistance to Pst. (a) The third leaves of wheat cultivar Shumai126 were inoculated with BSMV:γ, BSMV:PDS, BSMV:TaZFP8-1as and BSMV:TaZFP8-2as vectors. The virus symptoms (BSMV:γ and BSMV:TaZFP8) and photobleaching phenotypes (BSMV:PDS) on leaves were observed and photographed 10 days after infection. Mock, wheat leaves inoculated with FES buffer. (b) The resistance phenotype of the fifth leaves at 14 dpi, inoculated with Pst CYR34. (c-e) Relative expression of TaZFP8, TaPR1, TaPR2, TaRboh, TaCAT, and TaSOD in wheat leaves at 1 dpi. The transcript levels of the genes were detected by qRT-PCR. Error bars represent SEM from three independent biological replicates. Asterisks represent the level of significant differences. *p < 0.05, **p < 0.01, and ***p < 0.001