Fig. 3.

TaZFP8-5B negatively regulates plant disease resistance. (a and b) Overexpression of TaZFP8-5B increases wheat susceptibility to stripe rust. Fielder and TaZFP8-5B OE lines (TaZFP8-5B OE-J#2 and TaZFP8-5B OE-J#12) were inoculated with Pst CYR23, and disease symptoms were observed at 16 dpi (a), and fungal biomass in infected leaves was measured by qPCR (b). (c-e) Overexpression of TaZFP8-5B reduces rice resistance to Magnaporthe oryzae. (c) Disease symptoms of TaZFP8-5B OE lines (TaZFP8-5B OE-#1 and TaZFP8-5B OE-#2) and control. (d) Lesion length and (e) relative fungal growth were measured on leaves at 7 dpi. Error bars represent SEM from three independent biological replicates. Asterisks represent significant differences. ***p < 0.001