Fig. 4.
Transcription activity assay of full-length or truncation of TaZFP8-5B in yeast. (a) Illustration of pGBKT7-TaZFP8-5B (full-length) and pGBKT7-TaZFP8-5BΔEAR (EAR motif deletion mutant), and pGBKT7 constructs. (b) The constructs were separately transformed into yeast strain Y2HGold and diluted yeast solutions were dropped onto SD/-Leu/-Trp, and then onto SD/-Leu/-Trp/-His/X-α-gal media, respectively. Photographs were recorded after 2-d of incubation. All experiments were repeated three times with consistent results