Fig. 2. Epitope binning.

a A set of SEC-MALS experiments showing that VHH_VcP #1 and #2 can simultaneously bind to VcSiaP, forming a ternary complex. b A set of SEC-MALS experiments showing that at most three different VHHs bind to HiSiaP. Together with the data shown in Supplementary Fig. 2, these experiments revealed that VHH_HiP #9 and VHH_HiP #11 bind to separate epitopes, while VHH_HiP #4, 5, 6, 10 share an overlapping epitope. The epitope binning experiment was performed once (n = 1). The asterisk denotes a peak that resulted from VHH_HiP#8, which was excluded from further analyses because it did not bind to HiSiaP.