Fig. 5. Screening a cellular metabolism modulators library with SPACe analysis.

A Analysis of the library compounds induced toxicity represented as heatmap where cell count was normalized to DMSO, set as 1; the compounds represented caused a cell loss of >50% in at least one cell line. B Heatmap and hierarchical clustering of hits (Euclidean distance >2 in at least one cell line) from the screen after SPACe analysis. C 20× zoomed in images from screen run number 1 of the indicated hits shown in U-2 OS and PANC-1 cells. Scale bar: 10 µm. D Interpretability stacked bar graphs for the compounds shown in (C) with increased features in red, decreased in blue, and unchanged in gray. Source data are provided as a Source Data file.