Table 1.
The condition of Gap PCR.
| Procedure | Temperature (°C) | Time | Cycle number |
|---|---|---|---|
| Unchain/activate | 96 | 1min | 1 |
| Amplification | 96 | 10s | 25 |
| 50 | 10s | ||
| 60 | 2min | ||
| Keep warm | 16 | ∞ | 1 |
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