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. 2024 Nov 6;29:101327. doi: 10.1016/j.mtbio.2024.101327

Fig. 3.

Fig. 3

In vitro anti-biofilm and penetration performances. (A) 3D and 2D images of SYTO 9-stained MRSA biofilm after various treatments. (B) Schematic diagram of anti-biofilm mechanisms of PZH nanosystem. (C) Images of residue MRSA in biofilm spread on agar plates. (D) The number of viable MRSA bacteria in biofilm after treatments (n = 3). Note: ####p < 0.0001 vs PZH + NIR. (E) The biofilm bacterial viability relative to the control group (n = 3). Note: #p < 0.05 vs PZH + NIR, ####p < 0.0001 vs PZH + NIR. (F) The penetration of free drug or different nanoparticles into MRSA biofilm (scale bar: 30 μm), and the corresponding colocalization curve in each group: a) free ICG, b) ZI, c) PZ, d) PZH.