Figure 1.

mRNA for Nox2 is increased in platelets from aged mice. Levels of mRNA were measured in bead-purified platelets using quantitative polymerase chain reaction for catalytic subunits (A) Nox2, (B) Nox4, and (C) Nox1, and regulatory subunits (D) p47Phox and (E) p67Phox of NADPH oxidase and for Nox2-associated subunit (F) p22Phox. Relative expression to young Nox2 wild-type (Nox2-WT) is presented. Data are presented as median with IQR and analyzed using the Kruskal–Wallis test with Dunn’s multiple comparisons. N = 6 per group. Nox2-KO, Nox2 knockout.