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. 2024 Oct 18;8(7):102597. doi: 10.1016/j.rpth.2024.102597

Figure 2.

Figure 2

Protein levels of NADPH oxidase subunits or enzymatic activity in platelets are not altered by Nox2 genotype or age. Protein levels were measured in bead-purified platelets by Western blotting for catalytic subunits (A) Nox1 and (B) Nox4 and regulatory subunits (C) p47Phox and (D) p67Phox of NADPH oxidase. The activity of NADPH oxidase was measured in platelet lysate in the presence of cytochrome C and NADPH, and changes in absorbance in the presence or absence of superoxide dismutase were calculated and expressed as the amount of superoxide generated. Data are presented as median with IQR for A and C and as mean ± SE for B, D, and E. Data were analyzed using the Kruskal–Wallis test with Dunn’s multiple comparisons. N = 4 to 8 per group. AKO; aged Nox2 knockout; AW; aged Nox2 wild-type; Nox2-KO, Nox2 knockout; Nox2-WT, Nox2 wild-type; YKO; young Nox2 knockout; YW, young Nox2 wild-type.