Fig. 2.

Immunohistochemical assessments of tau pathology, reactive astrocytes, and neurons in PS19 mice. a Overview of sections from PS19 mice showing pTau (AT8, red), astrocytes (GFAP, purple), and neurons (MAP2, green), as well as merged images of the cortex (CTX, motor cortex, and somatosensory cortex), hippocampus (HPC), cerebellum (CBL), and brainstem (BS). Scale bar = 100 µm. b Quantitative comparison of AT8 occupancy in target regions between wild-type (WT) and PS19 mice, as well as a comparison of PS19 AT8 occupancy across target regions. c Quantitative comparison of GFAP occupancy in target regions between wild-type (WT) and PS19 mice, as well as a comparison of PS19 GFAP occupancy across target regions. d High-magnification images of sections from PS19 mice showing pTau (AT8, red), astrocytes (GFAP, purple), and neurons (MAP2, green), as well as merged images of the cortex (CTX), hippocampus (HPC), cerebellum (CBL), and brainstem (BS). Scale bar = 20 µm. e Quantitative comparison of AT8 occupancy between neurons (MAP2-positive) and astrocytes (GFAP-positive) in target regions of PS19 mice. CTX cortex, HIP hippocampus, PFC prefrontal cortex, BS brainstem, CBL cerebellum