Figure 2.

CXCR4 antagonism mobilizes neutrophils in the peripheral blood and preserves their function. (A) Absolute counts of mature neutrophils in the blood and (B) granulocyte subset cell counts in BM were determined 3 hours after the last dose on day 7. (C) Blood neutrophils were harvested 3 hours after the last dose on day 7, incubated with pHrodo BioParticles, and phagocytotic uptake was measured. (D) Blood neutrophils were harvested at 3 hours after the last dose on day 7, stimulated with phorbol 12-myristate 13-acetate, and ROS formation was measured. Data (mean + SEM) were from two independent experiments with 6–10 mice per group (A, B) and from one experiment with three to four mice per group (C, D). Statistics were calculated using the nonparametric Mann-Whitney test, two-sided. *p < 0.05, **p < 0.01, *** p < 0.001. BM, bone marrow; CXCR4, C-X-C chemokine receptor 4; ROS, reactive oxygen species; SEM, standard error of the mean; Veh., vehicle; WT, wild-type.