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. Author manuscript; available in PMC: 2024 Nov 25.
Published in final edited form as: Neuron. 1999 Sep;24(1):253–260. doi: 10.1016/s0896-6273(00)80837-5

Figure 1.

Figure 1.

Gene Targeting of Mouse PKCϵ

(A) Schematic structures of wild-type and recombinant loci, the targeting vector, and the 5’ probe used for screening ES cell clones and mice.

(B) Southern blot of genomic DNA following digestion with ApaI and ScaI, showing fragments corresponding to wild-type (4.3 kb) and targeted (1.6 kb) alleles.

(C) Western blots of DRG tissue from wild-type (+/+) and PKCϵ mutant (−/−) mice, using antibodies to PKC isozymes. Three immunoreactive bands of 65, 74, and 84 kDa were detected with the anti-PKCζ antibody. The 74 kDa band corresponds to what was reported for rat brain PKCζ (Hunter et al., 1995).

(D) Spontaneous locomotor activity and habituation of wild-type (white circles) and PKCϵ mutant (black circles) mice over 1 hr. Data are mean ± SE values from six mice of each genotype.