| Genetic reagent (M. musculus) |
C;129S4-Rag2tm1.1Flv Csf1tm1(CSF1)Flv Il2rgtm1.1Flv/J
|
PubMed:21791433 |
MGI:J:177073 |
RRID:IMSR_JAX:017708
|
| Genetic reagent (Homo sapiens) |
KYOUDXR0109B
|
ATCC |
ACS-1023 |
Human-induced pluripotent stem cells (iPSCs) |
| Genetic reagent (Homo sapiens) |
NCRM6
|
NHLBI |
NCRM6 (female) iPSC line |
From CD34+ cells, Episomal vectors |
| Genetic reagent (Homo sapiens) |
MS19-ES-H
|
NHLBI |
MS19-ES-H (female) iPSC line |
From PBMS cells, Cytotune Sendai Virus kit |
| Genetic reagent (Homo sapiens) |
NCRM5-AAVS1-CAG-EGFP
|
NHLBI |
NCRM5-AAVS1-CAG-EGFP (clone 5) |
From CD34+ cells, NCRM5 (male) reporter iPSC line with CAG-EGFP targeted mono-allelically at AAVS1 safe harbor |
| Genetic reagent (Homo sapiens) |
ND2-AAVS1-iCAG-tdTomato
|
NHLBI |
ND2-AAVS1-iCAG-tdTomato (clone 1) |
From fibroblast cells, ND2 (male) reporter iPSC line with insulated CAG-tdTomato targeted mono-allelically at AAVS1 safe harbor |
| Antibody |
anti-Iba1 (rabbit polyclonal) |
Wako |
Cat. #: 019-19741, RRID:AB_839504
|
IHC (1:500) |
| Antibody |
anti-human TMEM119 (rabbit polyclonal) |
Sigma-Aldrich |
Cat. #: HPA051870, RRID:AB_2681645
|
IHC (1:100) |
| Antibody |
anti-human CD68 (mouse monoclonal) |
R&D |
Cat. #: MAB20401 |
IHC (1:100) |
| Antibody |
anti-human CD45 (mouse monoclonal) |
R&D |
Cat. #: FAB1430R |
IHC (1:100) |
| Antibody |
anti-human CD11b (mouse monoclonal) |
R&D |
Cat. #: FAB1699R |
IHC (1:100) |
| Antibody |
anti-human CX3CR1 (rat monoclonal) |
Invitrogen |
Cat. #: 61-6099-42 |
IHC (1:100) |
| Antibody |
anti-human HLA (mouse monoclonal) |
Invitrogen |
Cat. #: 11-9983-42 |
IHC (1:100) |
| Antibody |
anti-mouse CD11b (rat monoclonal) |
Bio-Rad |
Cat. #: MCA711G |
IHC (1:100) |
| Antibody |
anti-GFAP (rat monoclonal) |
Invitrogen |
Cat. #: 13-0300, RRID:AB_2532994
|
IHC (1:200) |
| Antibody |
anti-mouse TMEM119 (guinea pig polyclonal) |
Synaptic systems |
Cat. #: 400 004, RRID:AB_2832239
|
IHC (1:500) |
| Antibody |
anti-CD68 (rat monoclonal) |
Bio-Rad |
Cat. #: MCA1957, RRID:AB_322219
|
IHC (1:200) |
| Antibody |
anti-CD34 (rat monoclonal) |
eBioscience |
Cat. #: 14-0341 |
IHC (1:30) |
| Antibody |
anti-PU.1 (rabbit monoclonal) |
Thermo Fisher Scientific |
Cat. #: MA5-15064 |
IHC (1:200) |
| Antibody |
anti-Trem2 (rabbit monoclonal) |
Thermo Fisher Scientific |
Cat. #: 702886 |
IHC (1:100) |
| Antibody |
anti-CD45 (rat monoclonal) |
Bio-Rad |
Cat. #: MCA1388, RRID:AB_321729
|
IHC (1:100) |
| Antibody |
anti glutamine synthetase (mouse monoclonal) |
Millipore |
Cat. #: MAB302, RRID:AB_2110656
|
IHC (1:200) |
| Antibody |
anti-RBPMS (guinea Pig polyclonal Ab) |
Phosphosolutions |
Cat. #: 1832-RBPMS |
IHC (1:100) |
| Antibody |
anti-cone arrestin (rabbit polyclonal) |
Millipore |
Cat. #: AB15282, RRID:AB_1163387
|
IHC (1:200) |
| Antibody |
anti-calbindin (rabbit polyclonal) |
Swant |
Cat. #: CB-38a |
IHC (1:5000) |
| Antibody |
anti-PKCa (rabbit polyclonal) |
Sigma-Aldrich |
Cat. #: P4334, RRID:AB_477345
|
IHC (1:200) |
| Antibody |
anti-RFP (rabbit polyclonal) |
RockLand |
Cat. #: 600-401-379-RTU |
IHC (1:100) |
| Antibody |
anti-Ki67-660 (rat monoclonal) |
eBioscience |
Cat. #: 50-5698-82, RRID:AB_2574235
|
IHC (1:50) |
| Antibody |
anti-P2RY12 (rabbit polyclonal) |
Thermo Fisher Scientific |
Cat. #: PA5-77671, RRID:AB_2736305
|
IHC (1:100) |
| Antibody |
anti-P2RY12 (rabbit polyclonal) |
Sigma-Aldrich |
Cat. #: HPA014518, RRID:AB_2669027
|
IHC (1:100) |
| Antibody |
Goat anti-Rabbit IgG Alexa Fluor 488 |
Invitrogen |
Cat. #: A27034, RRID:AB_2536097
|
IHC (1:200) |
| Antibody |
Goat anti-Rabbit IgG Alexa Fluor 568 |
Invitrogen |
Cat. #: A11011, RRID:AB_143157
|
IHC (1:200) |
| Antibody |
Goat anti-Rabbit IgG Alexa Fluor 647 |
Invitrogen |
Cat. #: A32733, RRID:AB_2633282
|
IHC (1:200) |
| Antibody |
Goat anti-mouse IgG Alexa Fluor 488 |
Invitrogen |
Cat. #: A28175, RRID:AB_2536161
|
IHC (1:200) |
| Antibody |
Goat anti-mouse IgG Alexa Fluor 568 |
Invitrogen |
Cat. #: A-11004, RRID:AB_141371
|
IHC (1:200) |
| Antibody |
Goat anti-mouse IgG Alexa Fluor 647 |
Invitrogen |
Cat. #: A-21235, RRID:AB_141693
|
IHC (1:200) |
| Antibody |
Donkey anti-Rat IgG Alexa Fluor 488 |
Invitrogen |
Cat. #: A-21208, RRID:AB_141709
|
IHC (1:200) |
| Antibody |
Donkey anti-Rat IgG Alexa Fluor 594 |
Invitrogen |
Cat. #: A-21209, RRID:AB_2535795
|
IHC (1:200) |
| Antibody |
Donkey anti-Rat IgG Alexa Fluor 650 |
Invitrogen |
Cat. #: SA5-10029, RRID:AB_2556609
|
IHC (1:200) |
| Antibody |
Rat monoclonal anti CD11b, Alexa Fluor 488 |
eBioscience |
Cat. #: 53-0112-82, RRID:AB_469901
|
IHC (1:50) |
| Peptide, recombinant protein |
human M-CSF |
Invitrogen |
Cat. #: PHC9501 |
|
| Peptide, recombinant protein |
Human IL3 |
R&D |
Cat. #: 203-IL-100 |
|
| Peptide, recombinant protein |
human IL-34 |
Peprotech |
Cat. #: 200–34 |
|
| Peptide, recombinant protein |
human CX3CL1 |
Peprotech |
Cat. #: 300–31 |
|
| Peptide, recombinant protein |
human TGFb1 |
R&D |
Cat. #: 7666-MB-005 |
|
| Peptide, recombinant protein |
human TGFb2 |
R&D |
Cat. #: 7346-B2-005 |
|
| Peptide, recombinant protein |
human BMP-4 |
GIBCO |
Cat. #: PHC9534 |
|
| Peptide, recombinant protein |
human SCF |
Miltenyi Biotec |
Cat. #: 130096692 |
|
| Peptide, recombinant protein |
human VEGF |
GIBCO |
Cat. #: PHC9394 |
|
| Chemical compound |
PLX5622 |
Plexxikon |
PLX5622 was provided by Plexxikon Inc and formulated in AIN-76A standard chow by Research Diets Inc |
1200 mg/kg in chow |
| Chemical compound |
NaIO3
|
Sigma-Aldrich |
Cat. #: S4007 |
|
| Chemical compound |
BSA |
Sigma-Aldrich |
Cat. #: A2153 |
|
| Chemical compound |
FBS |
Thermo Fisher Scientific |
Cat. #: A3160702 |
|
| Chemical compound |
Ketamine |
Anased |
Cat. #: NDC13985-584-10 |
|
| Chemical compound |
Xylazine |
Anased |
Cat. #: NDC59399-110-20 |
|
| Chemical compound |
Topical tropicamide |
Alcon |
Cat. # 215340 |
|
| Chemical compound |
Phenylephrine |
Alcon |
Cat. l# 215664 |
|
| Chemical compound |
0.5% Proparacaine HCL |
Sandoz |
Cat. #: 101571 |
|
| Chemical compound |
Surcrose |
Sigma-Aldrich |
Cat. #: S7903-5KG |
|
| Chemical compound |
OCT |
Thermo Fisher Scientific |
Cat. #: 23-730-571 |
|
| Chemical compound |
Fluorescein AK-FLUOR |
Akorn |
Cat. #: 17478-253-10 |
|
| Chemical compound |
Tamoxifen |
Sigma-Aldrich |
Cat. #: T5648-5G |
|
| Chemical compound |
HBSS |
Sigma-Aldrich |
Cat. #: H8264-1L |
|
| Chemical compound |
L-(+)-Cysteine hydrochloride monohydrate |
Fisher |
Cat. #: C562-25 |
|
| Chemical compound |
Papain, lyophilized |
Worthington Biochemical |
Cat. #: LS003119
|
|
| Chemical compound |
DNAse I |
Worthington Biochemical |
Cat. #: LS006333
|
|
| Chemical compound |
Superoxide dismutase |
Worthington Biochemical |
Cat. #: LS003540
|
|
| Chemical compound |
Catalase |
Sigma-Aldrich |
Cat. #: C1345-1G |
|
| Chemical compound |
(+)-α-Tocopherol acetate |
Sigma-Aldrich |
Cat. #: T-1157-1G |
|
| Chemical compound |
Gentamicin solution |
Sigma-Aldrich |
Cat. #: G1397-10ml |
|
| Chemical compound |
D-(+)-Glucose |
Sigma-Aldrich |
Cat. #: G7021-100g |
|
| Chemical compound |
Antipain dihydrochloride |
Roche |
Cat. #: 11004646001 |
|
| Chemical compound |
HEPES |
Invitrogen |
Cat. #: 15630080 |
|
| Chemical compound |
EDTA |
KD medical |
Cat. #: RGC-3130 |
|
| Chemical compound |
RNAlater solution |
Ambion |
Cat. #: AM7021 |
|
| Chemical compound |
Triton X-100 |
Sigma-Aldrich |
Cat. #: X100100ml |
|
| Chemical compound |
Tween 20 |
Sigma-Aldrich |
Cat. #: P1379-100ml |
|
| Chemical compound |
Paraformadehyde |
Fisher Scientific |
Cat. #: 50-259-97 |
|
| Chemical compound |
Donkey serum |
Sigma-Aldrich |
Cat. #: D9663-10ml |
|
| Chemical compound |
Goat serum |
Sigma-Aldrich |
Cat. #: G9023-10ml |
|
| Commercial assay or kit |
Bloking Reagent |
Sigma-Aldrich |
Cat. #: 11096176001 |
|
| Commercial assay or kit |
In Situ Cell Death Detection Kit, TMR red |
Sigma-Aldrich |
Cat. #: 12156792910 |
|
| Commercial assay or kit |
Ib4 Alexa Fluor 568 |
Invitrogen |
Cat. #: I21412
|
IHC (1:200) |
| Commercial assay or kit |
Ib4 Alexa Fluor 647 |
Invitrogen |
Cat. #: I32450
|
IHC (1:200) |
| Commercial assay or kit |
DAPI |
Sigma-Aldrich |
Cat. #: D9542 |
IHC (1:200) |
| Commercial assay or kit |
Mounting medium without DAPI |
Vector |
Cat. #: H-1000 |
|
| Commercial assay or kit |
Mounting medium with DAPI |
Vector |
Cat. #: H-1200 |
|
| Commercial assay or kit |
RNeasy Mini Kit |
QIAGEN |
Cat. #: 74104 |
|
| Commercial assay or kit |
Rnase free Dnase set |
QIAGEN |
Cat. #: 79254 |
|
| Commercial assay or kit |
First strand cDNA synthesis |
Takara |
Cat. #: 6110A |
|
| Commercial assay or kit |
MessageBooster cDNA synthesis kit |
Epicentre |
Cat. #: MB060110
|
|
| Commercial assay or kit |
Fast SYBR Green Master Mix |
Thermo Fisher Scientific |
Cat. #: 4385617 |
|
| Commercial assay or kit |
LiDirect Lightening genotyping kit |
LifeSci |
Cat. #: M0015 |
|
| Commercial assay or kit |
eBioscience Flow Cytometry Staining Buffer |
Thermo Fisher Scientific |
Cat. #: 00-4222-57 |
|
| Commercial assay or kit |
X-VIVO-15 medium |
Lonza |
Cat. #: BEBP02-061Q |
|
| Commercial assay or kit |
DMEM:F12 medium |
Thermo Fisher Scientific |
Cat. #: 11330057 |
|
| Commercial assay or kit |
mTeSR1 |
Stemcell technologies |
Cat. #: 85850 |
|
| Commercial assay or kit |
N2 supplement |
Thermo Fisher Scientific |
Cat. #: 17502048 |
|
| Commercial assay or kit |
Non-essential Amino Acids (NEAA) |
Thermo Fisher Scientific |
Cat. #: 11140050 |
|
| Commercial assay or kit |
GlutaMax Supplement |
Thermo Fisher Scientific |
Cat. #: 35050061 |
|
| Commercial assay or kit |
Geltrex |
Thermo Fisher Scientific |
Cat. #: A1413301 |
|
| Commercial assay or kit |
TrypLE Express |
Thermo Fisher Scientific |
Cat. #: 12605010 |
|
| Commercial assay or kit |
Rho-kinase inhibitor Y-27632 |
abcam |
Cat. #: ab143784 |
|
| Commercial assay or kit |
mFreSR |
Stemcell technologies |
Cat. # 05854 |
|
| Commercial assay or kit |
Stem Cell Dissociation Reagent |
ATCC |
Cat, #: ACS-3010 |
|
| Commercial assay or kit |
Stem Cell Freezing Media |
ATCC |
Cat. #: ACS-3020 |
|
| Commercial assay or kit |
Penicillin-Streptomycin |
Thermo Fisher Scientific |
Cat. #: 15140122 |
|
| Commercial assay or kit |
pHrodo Red E. coli BioParticles |
Thermo Fisher Scientific |
Cat. #: P35361
|
|
| Commercial assay or kit |
pHrodo Red Zymosan Bioparticles |
Thermo Fisher Scientific |
Cat. #: P35364
|
|
| Commercial assay or kit |
Bovine rod outer segment |
Invision Bioresources |
Cat. #: 98740 |
|
| Commercial assay or kit |
Vybrant DiI Cell-Labeling Solution |
Thermo Fisher Scientific |
Cat. #: V22885 |
|
| Commercial assay or kit |
Lipopolysaccharides (LPS) |
Sigma-Aldrich |
Cat. #: L2630 |
|
| Commercial assay or kit |
RIPA lysis buffer |
Sigma-Aldrich |
Cat. #: R0278 |
|
| Commercial assay or kit |
proteinase inhibitor mixture |
Calbiochem |
Cat. #: 539132 |
|
| Commercial assay or kit |
Pierce BCA Protein Assay Kit |
Thermo Fisher Scientific |
Cat. #: 23227 |
|
| Commercial assay or kit |
Milliplex bead assay kit |
Millipore |
Cat. #: MCYTOMAG-70K |
|
| Commercial assay or kit |
AggreWellsTM800 |
Stemcell technologies |
Cat. #: 34825 |
|
| Software |
ImageJ |
ImageJ (http://imagej.nih.gov/ij/) |
RRID:SCR_003070
|
|
| Software |
GraphPad Prism7 |
GraphPad Prism (https://graphpad.com) |
RRID:SCR_015807
|
Version 7 |
| Software |
IPA |
QIAGEN |
RRID:SCR_008653
|
|
| Software |
JMP |
JMP |
RRID:SCR_014242
|
Version 12 |
