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. 2024 Nov 25;19:88. doi: 10.1186/s13024-024-00779-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Generation of CTSB, CTSD and CTSL knockdown cell lines. A Validation of target knockdown in CRISPRi RPE1 cells. CTSB, CTSD and CTSL mRNA levels were measured by qPCR in control (CRISPRi Ctl), CTSB-knockdown (CTSBi), CTSD-knockdown (CTSDi) and CTSL-knockdown (CTSLi) cell lines. B CatB protein levels in control, CTSBi, CTSDi and CTSLi cell lines. C CatD protein levels in control, CTSBi, CTSDi and CTSLi cell lines. D CatL protein levels in control, CTSBi, CTSDi and CTSLi cell lines. E Protein levels of CatB in CRISPRi control, CRISPRa control, CTSB knockdown (CTSBi) and CTSB upregulation (CTSBa) RPE1 cell lines. F CatB activity measured by fluorogenic assay. G-I CatB, CatD and CatL protein levels in control and CTSBa cells