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. 2024 Nov 25;24:1117. doi: 10.1186/s12870-024-05850-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 8 — Verification of the interaction of PbELF4a and PbELF4b with PbELF3α proteins. (A) and (B) Yeast two-hybrid assays examining the interaction of PbELF4a and PbELF4b with PbELF3α proteins. Plasmid combinations were transferred into yeast strains, and interactions between prey and bait were tested on synthetic dropout (SD) media. SD-LW indicates SD media lacking Leu and Trp, while SD-LWHA indicates SD media lacking Leu, Trp, His, and Ade. (C) and (D) Firefly luciferase complementation imaging assays testing the interaction between PbELF4a and PbELF4b with PbELF3α proteins. Agrobacterium strain combinations were infected to the corresponding areas of tobacco leaves, with luminescence intensity depicted by the pseudo color bar