Figure 2.

Methodology to uncover candidate proteins that affect RNA folding in vivo. A library of E. coli MG1655 cells with random transposon insertions of the TetR cassette was generated by adapting previously published procedures (77). The plasmid containing the iRS [3] system with the probe 3 asRNA was transformed into the library. Cells were sorted based on their GFP expression. High-fluorescence isolates were prepped for whole-genome sequencing, allowing for transposon insertion mapping. Image created with BioRender.com.