FIG. 4.

Point mutation of both NLS2 and NLS3 abrogates nuclear localization of VP13/14. (A) Schematic diagram of the GFP-13/14 fusion protein (Wt) and the mutants in which arginine residues have been mutated to glycine residues for NLS2 alone (ΔNLS2), NLS3 alone (ΔNLS3), or both NLS2 and NLS3 (ΔNLS2+3). The clusters of arginine residues are shown as solid boxes. (B) The four constructs diagrammed in panel A were transfected into COS-1 cells and examined live by confocal microscopy 40 h after transfection.