FIG. 6.

VP13/14 is capable of nuclear shuttling. Heterokaryon assays were carried out between mouse NIH 3T3 cells and monkey COS-1 cells expressing either Myc-hnRNPA1 (A), Myc-hnRNPC1 (B), SV5-13/14 (C), or GFP-13/14 (D). In each case the cells were fixed and stained with either anti-Myc (A and B), anti-SV5 tag (C), or anti-GFP (D) antibodies. In the case of GFP-13/14, the cells were also examined for intrinsic GFP fluorescence (D, GFP fl.). Mouse cells (arrowed in all examples) were identified by their speckled nuclei when stained with DAPI.