Table 1.
name | buffer | Tm (°C) for 10−5 M | ΔHapp (kcal/mol) | ΔSapp (cal/mol) | ΔG37 (kcal/mol) | ΔΔG37 (kcal/mol) |
---|---|---|---|---|---|---|
4T d3WJ | CB1 | 43.9 ± 1.2 | 89.6 | −257.1 | −9.88 | −2.79 |
CB2 | 33.9 ± 0.8 | −96.2 | −287.4 | −7.09 | ||
3T d3WJ | CB1 | 43.3 ± 0.7 | −104.8 | −305.1 | −10.23 | −3.08 |
CB2 | 34.9 ± 0.6 | −111.5 | −336.5 | −7.15 | ||
2T d3WJ | CB1 | 44.3 ± 0.6 | −131.1 | −387.3 | −11.00 | −3.41 |
CB2 | 36.1 ± 0.6 | −94.4 | −279.7 | −7.59 | ||
1T d3WJ | CB1 | 43.2 ± 0.7 | −137.8 | −410.6 | −10.50 | −2.94 |
CB2 | 35.2 ± 0.5 | −99.2 | −295.5 | −7.56 | ||
0T d3WJ | CB1 | 41.1 ± 0.5 | −71.1 | −200.2 | −9.03 | −1.3 |
CB2 | 33.5 ± 0.4 | −43.4 | −115.2 | −7.73 |
The errors in enthalpy, entropy, and free energy are estimated to be 10%, 10%, and 5%, respectively.49