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. 2024 Nov 25;13:RP98747. doi: 10.7554/eLife.98747

Figure 3. Loss of Notch activity in the sebaceous gland (SG) stem cells inhibits sebocyte differentiation.

(a, b) Representative co-stain images for N1ICD and fatty acid synthase (FASN) in SGs from mice (n=5 each) treated with aRW (a) and aJ2 (b), 3 d post-treatment. (c, d) Representative adipophiin staining in SGs from mice (n=5 each) treated with aRW (c) and aJ2 (d), 3 d post-treatment. (e, f) Representative adipophilin staining in SGs from mice (n=5 each) treated with aRW (e) and aJ2 (f), 7 d post-treatment. (g) A representative hematoxylin and eosin (H&E) image of an SG from mice (n=5 each) treated with aJ2, 7 d post-treatment. Arrow points to a bursting sebocyte releasing sebum into the sebaceous duct. (h) Quantification of the number of cells expressing adipophilin in each SG, 3 d post-treatment with aRW, aJ1, and aJ2. p-values: aJ1=0.749, aJ2=0.062. Total n of SGs quantified per treatment: aRW = 17, aJ1=14, aJ2=30. (i) Quantification of the number of cells expressing adipophilin in each SG, 7 d post-treatment with aRW, aJ1, aJ2, and aJ1J2. p-values: aJ1=0.301, aJ2=4.13E-14, aJ1J2=4.06E-14. Total n of SGs quantified per treatment: aRW = 18, aJ1=19, aJ2=22, aJ1J2=21. Student’s t-test used for statistical analysis. All treatments were compared against aRW. Error bars represent SEM. Scale bars are 25 μm.

Figure 3—source data 1. Source data for Figure 3.

Figure 3.

Figure 3—figure supplement 1. Loss of Notch activity in the sebaceous gland (SG) stem cells inhibits sebocyte differentiation.

Figure 3—figure supplement 1.

(Related to Figure 3). (a) A representative co-stain image for N1ICD and Fatty acid synthase (FASN) in an SG from mice (n=5 each) treated with aJ1, 3 d post-treatment. (b,c) Representative FASN staining in SGs from mice (n=5 each) treated with aRW (b) and aJ2 (c), 3 d post treatment. (d) A representative adipophilin staining in an SG from mice (n=5 each) treated with aJ1, 3 d post-treatment. (e) A representative FASN staining in an SG from mice (n=5 each) treated with aJ1, 3 d post-treatment. (f,g) Representative FASN staining in SGs from mice (n=5 each) treated with aRW (f) and aJ2 (g), 7 d post-treatment. (h) A representative adipophilin staining in an SG from mice (n=5 each) treated with aJ1, 7 d post-treatment. (i) A representative FASN staining in an SG from mice (n=5 each) treated with aJ1, 7 d post-treatment. (j) A representative adipophilin staining in an SG from mice (n=5 each) treated with aJ1J2, 7 d post treatment. (i) A representative FASN staining in an SG from mice (n=5 each) treated with aJ1J2, 7 d post treatment. Scale bars are 25 μm.