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. 2024 Nov 25;13:RP98747. doi: 10.7554/eLife.98747

Figure 4. Notch activity in the sebaceous gland (SG) stem cells is required to prevent unregulated progenitor proliferation.

(a,b) Representative co-stain images for Lrig1 and androgen receptor (AR) in SGs from mice (n=5 each) treated with aRW (a) and aJ2 (b), 3 d post-treatment. (c) A representative co-stain image for fatty acid synthase (FASN) and AR in an SG from mice (n=5 each) treated with aRW, 3 d post-treatment. Arrows point to sebocytes, and arrowheads point to progenitor cells. (d) Quantification of the number of cells expressing Lrig1 in each SG, 3 d post-treatment with aRW, aJ1. and aJ2. p-values: aJ1=0.152, aJ2=0.450. (e) Quantification of what percentage of the Lrig1 + cells express AR, 3 d post-treatment with aRW, aJ1, and aJ2. p-values: aJ1=0.789, aJ2=0.028. (d and e) Total n of SGs quantified per treatment: aRW = 17, aJ1=10, aJ2=24. (f,g) Representative co-stain images for Lrig1 and AR in SGs from mice (n=5 each) treated with aRW (f) and aJ2 (g), 7 d post-treatment. (h) Quantification of the number of cells expressing Lrig1 in each SG, 7 d after treatment with aRW, aJ1, aJ2, and aJ1J2. p-values: aJ1=0.628, aJ2=9.06E-07, aJ1J2=7.88E-06. (i) Quantification of what percentage of the Lrig1 + cells express AR, 7 d after treatment with aRW, aJ1, aJ2, and aJ1J2. p-values: aJ1=0.117, aJ2=0.248, aJ1J2=0.091. (h and i) Total n of SGs quantified per treatment: aRW = 12, aJ1=12, aJ2=18, aJ1J2=16. (j,k) Representative co-stain images for Ki67 and FASN in SGs from mice (n=5 each) treated with aRW (j) and aJ2 (k), 7 d post-treatment. (l) Quantification of the number of cells expressing Ki67 in each SG, 7 d after treatment with aRW, aJ1, and aJ2. p-values: aJ1=0.652, aJ2=0.009. Total n of SGs quantified per treatment: aRW = 11, aJ1=12, aJ2=16. Student’s t-test used for statistical analysis. All treatments were compared against aRW. Error bars represent SEM. Scale bars are 25 μm.

Figure 4—source data 1. Source data for Figure 4.

Figure 4.

Figure 4—figure supplement 1. Notch activity in the sebaceous gland (SG) stem cells is required to prevent unregulated progenitor proliferation.

Figure 4—figure supplement 1.

(Related to Figure 4). (a,b) Representative co-stain images for Lrig1 and androgen receptor (AR) in SGs from mice (n=5 each) treated with aRW (a) and aJ1 (b), 3 d post-treatment. (c–e) Representative co-stain images for Lrig1 and AR in SGs from mice (n=5 each) treated with aRW (c), aJ1 (d), and aJ1J2 (e), 7 d post-treatment. (f,g) Representative co-stain images for Ki67 and fatty acid synthase (FASN) in SGs from mice (n=5 each) treated with aRW (f) and aJ1 (g), 7 d post-treatment. Scale bars are 25 μm.