Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (Mus musculus) | Dorsal skin tissue | Charles River-Hollister | Females | |
| Antibody | Anti-Jag1 (Mouse monoclonal) | Genentech | Inhibiting antibody (20 mg/kg) |
|
| Antibody | Anti-Jag2 (Mouse monoclonal) | Genentech | Inhibiting antibody (20 mg/kg) |
|
| Antibody | Anti-Notch1 (Human monoclonal) | Genentech | Inhibiting antibody (5 mg/kg) |
|
| Antibody | Anti-Notch2 (Mouse monoclonal) | Genentech | Inhibiting antibody (10 mg/kg) |
|
| Antibody | Anti-Ragweed (Mouse monoclonal) | Genentech | Isotype control antibody – concentrations match the maximum dose of inhibiting antibodies | |
| Antibody | Anti-N1ICD (Rabbit monoclonal) | Cell Signaling | Cat#: 4147 | Triple stain (20 ug/ml) IF (1:500) |
| Antibody | Anti-FASN (Mouse monoclonal) | BD | Cat#: 610963 | IF (1:100) |
| Antibody | Anti-Adipophilin (Guinea pig polyclonal) | Fitzgerald | Cat#: 20R-AP002 | IF (1:500) |
| Antibody | Anti-Ki67 (Rabbit monoclonal) | Thermo Fisher Scientific | Cat#: SP6 RM-9106-SO | IF (1:100) |
| Antibody | Anti-Lrig1 (Goat polyclonal) | R&D Systems | Cat#: AF3688-SP | IF (1:200) |
| Antibody | Anti-AR (Rabbit monoclonal) | Abcam | Cat#: ab133273 [EPR1535(2)] | IF (1:250) |
| Antibody | PowerVision poly-HRP anti-rabbit (Goat polyclonal) | Leica | Cat#: PV6119 | ready to use |
| Sequence-based reagent | RNAScope LS 2.5 Murine-Jag2_C1 | ACD | Cat#: 417518 | nucleotides spanning from nt 552–1480 of reference sequence NM_010588.2 |
| Sequence-based reagent | RNAScope LS 2.5 Murine-Notch1_C2 | ACD | Cat#: 404648-C2 | nucleotides spanning from nt 1153–1960 of reference sequence NM_008714.3 |
| Sequence-based reagent | RNAScope LS 2.0 Murine-PPIB probe | ACD | Cat#: 313917 | nucleotides spanning from nt 98~856 of reference sequence NM_011149.2 |
| Sequence-based reagent | RNAScope LS 2.0 DapB probe | ACD | Cat#: 312038 | nucleotides spanning from nt 414~862 reference sequence EF191515 |
| Commercial assay or kit | RNAscope LS Multiplex Reagent Kit | ACD | Cat#: 322800 | |
| Commercial assay or kit | Bond Epitope Retrieval Solution 2 | Leica | Cat#: AR9640 | |
| Commercial assay or kit | Opal Polaris 7 Auto Detection Kit | Akoya | Cat#: NEL811001KT |