Table 1.
Species (common name) | Identification approach (Query/e-value) | WAK vs. WAKL: Protein domains used for classification | Reference |
---|---|---|---|
Brassica rapa (Chinese cabbage) | LBp & LBn (AtWAK & AtWAKL/< 0.01) | WAK: WAK or GWB &/or WAKa & EGF-like/EGF_CA & pk & TM | Zhang et al. (2020) |
WAKL: pk &/or WAK/GWB or EGF | |||
Cannabis sativa (Cannabis) | LBp (AtWAK & AtWAKL/< 1e-5) | WAK: GWB & EGF/EGF_CA & pk & SP & TM | Sipahi et al. (2022) |
WAKL: pk &/or GWB &/or EGF/EGF_CA &/or SP &/or TM | |||
Hordeum vulgare (Barley) | HMM + TBLASTn (EGF_CA & pk & GWB/< -100) |
N/A | Tripathi et al. (2021) |
Juglans mandshurica (Walnut) | LBp (AtWAK/< 1e-5) | WAK: WAK/GWB & STK/PKc, EGF/EGF_CA & SP & TM |
Li et al. (2022) |
Juglans regia (Walnut) | WAKL: WAK/GWB & STK/PKc, EGF/EGF_CA &/or SP &/or TM |
||
Malus domestica (Apple) | Not specified (EGF & pk) | WAK: EGF & TM & pk | Zuo et al. (2019) |
Medicago truncatula (Barrel medic) | BLASTp + HMM (AtWAK & OsWAK/< 1e-5) | WAK: EGF_CA & WAKa & GWB & pk_Tyr | Kong et al. (2023) |
Nicotiana benthamiana | HMM (AtWAK & AtWAKL & SlWAK & SlWAKL) | WAK: GWB & EGF & pk | Zhong et al. (2023) |
WAKL: GWB &/or EGF &/or pk (Two of three) | |||
Pisum sativum (Pea) | LBp (AtWAK/< 1e-5) | WAK: STK/PKc_like & GWB | Li et al. (2023) |
Rosa chinensis (Rose) | LBp (AtWAK & AtWAKL) + HMM (EGF_CA, GWB, pk_Ser-Thr/<1e-3) |
WAK: EGF_CA, GWB, pk, SP, TM | Liu et al. (2021) |
WAKL: SP, TM, pk &/or EGF_CA and/or GWB | |||
Saccharum spontaneum (Wild sugarcane) | HMM (GWB & EGF & pk) | WAK: GWB & EGF & pk | Wang et al. (2023) |
Sorghum bicolor (Sorghum) | |||
Sesame indicum (Sesame) | LBp (AtWAKLs/<10-10) | WAKL: GWB & pk | Yan et al. (2023) |
Solanum tuberosum (Potato) | HMM | WAK: GUB_WAK/WAKa/EGF & TM & pk | Yu et al. (2022) |
WAKL: TM & pk &/or GUB_WAK/WAKa/EGF | |||
Triticum aestivum (Bread wheat) | LBp (AtWAK) + HMM (WAK family) | WAK: GWB & TM & pk | Xia et al. (2022) |
Zea mays (Maize) | HMM (GWB & EGF_CA & pk/< 0.001) | WAKL: GWB & EGF_CA &/or pk | Hu et al. (2023) |
Gossypium hirsutum (Cotton) and Solanum lycopersicum (Tomato) summarized in Figure 1 .
HMM, Hidden Markov model search; LBp, Local BLASTp; LBn, Local BLASTn; pk, pkinase; GWB, GUB_WAK_bind; TM, Transmembrane; WAKa, WAK_assoc; STK, STKc_IRAK; PKc, PKc_Superfamily; SP, Signal peptide.
& = must include the previous and following domain.
Or = Must contain either the previous or following domain.