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. 2024 Nov 12;15:1467148. doi: 10.3389/fpls.2024.1467148

Table 1.

Methods and protein domains used for identification and classification of predicted WAK and WAKL proteins across various plant species.

Species (common name) Identification approach (Query/e-value) WAK vs. WAKL: Protein domains used for classification Reference
Brassica rapa (Chinese cabbage) LBp & LBn (AtWAK & AtWAKL/< 0.01) WAK: WAK or GWB &/or WAKa & EGF-like/EGF_CA & pk & TM Zhang et al. (2020)
WAKL: pk &/or WAK/GWB or EGF
Cannabis sativa (Cannabis) LBp (AtWAK & AtWAKL/< 1e-5) WAK: GWB & EGF/EGF_CA & pk & SP & TM Sipahi et al. (2022)
WAKL: pk &/or GWB &/or EGF/EGF_CA &/or SP &/or TM
Hordeum vulgare (Barley) HMM +
TBLASTn (EGF_CA & pk & GWB/< -100)
N/A Tripathi et al. (2021)
Juglans mandshurica (Walnut) LBp (AtWAK/< 1e-5) WAK: WAK/GWB & STK/PKc,
EGF/EGF_CA & SP & TM
Li et al. (2022)
Juglans regia (Walnut) WAKL: WAK/GWB & STK/PKc,
EGF/EGF_CA &/or SP &/or TM
Malus domestica (Apple) Not specified (EGF & pk) WAK: EGF & TM & pk Zuo et al. (2019)
Medicago truncatula (Barrel medic) BLASTp + HMM (AtWAK & OsWAK/< 1e-5) WAK: EGF_CA & WAKa & GWB & pk_Tyr Kong et al. (2023)
Nicotiana benthamiana HMM (AtWAK & AtWAKL & SlWAK & SlWAKL) WAK: GWB & EGF & pk Zhong et al. (2023)
WAKL: GWB &/or EGF &/or pk (Two of three)
Pisum sativum (Pea) LBp (AtWAK/< 1e-5) WAK: STK/PKc_like & GWB Li et al. (2023)
Rosa chinensis (Rose) LBp (AtWAK & AtWAKL) + HMM
(EGF_CA, GWB, pk_Ser-Thr/<1e-3)
WAK: EGF_CA, GWB, pk, SP, TM Liu et al. (2021)
WAKL: SP, TM, pk &/or EGF_CA and/or GWB
Saccharum spontaneum (Wild sugarcane) HMM (GWB & EGF & pk) WAK: GWB & EGF & pk Wang et al. (2023)
Sorghum bicolor (Sorghum)
Sesame indicum (Sesame) LBp (AtWAKLs/<10-10) WAKL: GWB & pk Yan et al. (2023)
Solanum tuberosum (Potato) HMM WAK: GUB_WAK/WAKa/EGF & TM & pk Yu et al. (2022)
WAKL: TM & pk &/or GUB_WAK/WAKa/EGF
Triticum aestivum (Bread wheat) LBp (AtWAK) + HMM (WAK family) WAK: GWB & TM & pk Xia et al. (2022)
Zea mays (Maize) HMM (GWB & EGF_CA & pk/< 0.001) WAKL: GWB & EGF_CA &/or pk Hu et al. (2023)

Gossypium hirsutum (Cotton) and Solanum lycopersicum (Tomato) summarized in Figure 1 .

HMM, Hidden Markov model search; LBp, Local BLASTp; LBn, Local BLASTn; pk, pkinase; GWB, GUB_WAK_bind; TM, Transmembrane; WAKa, WAK_assoc; STK, STKc_IRAK; PKc, PKc_Superfamily; SP, Signal peptide.

& = must include the previous and following domain.

Or = Must contain either the previous or following domain.