FIG. 3.

Activation of IFN-responsive genes by Stat1N-TAD fusions. U3A stable transfectants expressing wild-type Stat1 or various Stat1N-TAD fusions were selected. (A) Western blot analysis using an antibody against Stat1 N-terminal domain. (B) RT-PCR analysis as detailed in Materials and Methods was performed on the indicated endogenous genes from the Stat1N-TAD stable cell lines treated with IFN-α or IFN-γ or left untreated. In the samples of GAPDH (−RT), reverse transcriptase was left out in the reverse transcription.