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. 2024 Nov 8;25(12):2297–2307. doi: 10.1038/s41590-024-01999-3

Extended Data Fig. 7. MCT11 overexpression accelerates T cell dysfunction in the TME.

Extended Data Fig. 7

a) OT-I Thy1.1 T cells were retrovirally transduced with an MCT11 overexpression vector (OE) or control empty vector (EV), and 3 million T cells were adoptively transferred into day 7 B16OVA bearing C57BL/6 Thy1.2 mice. b) Representative flow plot and quantification of percentage of CD8+Thy1.1+ transduced with EV (n = 9) or OE (n = 10) (transferred OT-Is) infiltrating B16OVA tumors. c) Representative flow plot and quantification of PD1+Tim3+ population in adoptively transferred EV (n = 9) or OE (n = 10) OT-I T cells. d) Representative flow plot and quantification of TNF+IFNʏ+ population in adoptively transferred EV (n = 9) or OE (n = 10) T cells. Data represent three independent experiments. Error bars indicate +/− SEM. Statistical analysis performed by unpaired two-tailed Student’s T tests (b-d). (a) Created in BioRender. Peralta, R. (2024) BioRender.com/k52v484.

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