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. 2024 Nov 25;25(12):2270–2283. doi: 10.1038/s41590-024-02011-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — (a) Volcano plots showing DEGs due to OVA-IC stimulation in MHCII+CD11c− and MHCII+CD11c+ macrophages from wild type mice by RNA-seq. Wald test was used. (b) Volcano plots showing DEGs due to OVA-IC stimulation in Lyve1 + , MHCII+CD11c − , and MHCII+CD11c+ macrophages from Fcgr2b − /− mice by RNA-seq. Wald test was used. (c) Gene ontogeny (GO) analysis of DEGs specific to each macrophage subset with all the DEGs of 3 subsets as the background gene list. (d) Heat map of expression of chemokines with or without IV IC injection in MHCII+CD11c− and MHCII+CD11c+ macrophages from wild type and Fcgr2b − /− mice (scaled normalized values).