Extended Data Fig. 10. CGRP induces distinct transcriptional changes in MHCII+CD11c− and MHCII+CD11c+ macrophages.
(a) CGRP ELISA of DRG culture supernatants stimulated with supernatants from IC stimulated synovial explant. TNF neutralizing antibodies (4 μg/ ml) or isotype control were added to synovial supernatants before adding to DRG neurons. n = 4 mice for each group. Data represents mean ± SEM. Two-tailed t test was used. (b) Illustration of receptor activity-modifying protein (RAMP) association on calcitonin receptor-like receptor (CLR). (c) Expression of marker genes for macrophages and neuropeptide receptors in healthy synovium. scRNA-seq data from GSE145286. (d) Principle component analysis (PCA) of MHCII+CD11c− and MHCII+CD11c+ macrophages from synovial explant of WT mice treated with 100 nM CGRP or control for 4 hours by RNA-seq. n = 3 mice were compiled for each plot and n = 18 mice for each population. (e) Volcano plots showing differentially expressed genes in MHCII+CD11c− and MHCII+CD11c+ macrophages with CGRP stimulation. Wald test was used.