Table 1.
Primers used for qPCR.
| Primer | Accession number | Product size | Sequence (5′-3′) |
|---|---|---|---|
| Human_ ESR | XM_054354491 | 147 | F: TGCCCTACTACCTGGAGAAC |
| R: CCATAGCCATACTTCCCTTGTC | |||
| Human_ PGR | XM_054369129 | 106 | F: GAAGGGCAGCACAACTACTTA |
| R: CAGCCTGACAGCACTTTCTA | |||
| Human_ ITGβ1 | NM_033668 | 235 | F: TGTCCCACTGGTCCAGACA |
| R: GACCAGCTTTACGTCCGTAGTT | |||
| Human_ LIF | NM_002309 | 233 | F: TCTTGGCGGCAGGAGTTG |
| R: CTTGTCCAGGTTGTTGGGGA | |||
| Human_ PAEP | NM_001018049 | 296 | F: GCGACCAACAACATCTCCCT |
| R: GCCAGGTACTGGCACATCAT | |||
| Human_MKI67 | NM_001145966 | 736 | F: CTGACCCTGATGAGAGTGAGGGA |
| R: TCTCCCCTTTTGAGAGGCGT | |||
| Human_MUC1 | NM_001371720 | 119 | F: TGCTTACAGCTACCACAGCC |
| R: GCTGGGCACTGAACTTCTCT | |||
| Human_HSPA1B | NM_005346 | 131 | F: GCGAGGCGGACAAGAAGAA |
| R: GATGGGGTTACACACCTGCT | |||
| Human_GADD45A | NM_001924 | 145 | F: GAGAGCAGAAGACCGAAAGGA |
| R: CACAACACCACGTTATCGGG | |||
| Human_DDIT3 | NM_001195055 | 116 | F: GGAAACAGAGTGGTCATTCCC |
| R: CTGCTTGAGCCGTTCATTCTC | |||
| Human_DUSP1 | NM_004417 | 86 | F: GCCTTGCTTACCTTATGAGGAC |
| R: GGGAGAGATGATGCTTCGCC | |||
| Human_ ATF4 | NM_182810 | 153 | F: ATGACCGAAATGAGCTTCCTG |
| R: GCTGGAGAACCCATGAGGT | |||
| Human_ JUNB | NM_002229 | 128 | F: ACAAACTCCTGAAACCGAGCC |
| R: CGAGCCCTGACCAGAAAAGTA | |||
| Human_ MAPK | NM_001286124 | 168 | F: CGAGCCAGGCAGTGATTTGA |
| R: CAGTAACGAGGGAGGGCTTC | |||
| Human_IL8 | NM_000584 | 248 | F: TCTGCAGCTCTGTGTGAAGG |
| R: TTCTCAGCCCTCTTCAAAAACT | |||
| Human_ZO1 | XM_054378703 | 218 | F: CTCAAGAGGAAGCTGTGGGT |
| R: AATCCAGGAGCCCTGTGAAG | |||
| Human_ TNF-α | NM_000594 | 185 | F: CACAGTGAAGTGCTGGCAAC |
| R: AGGAAGGCCTAAGGTCCACT | |||
| Human_IL6 | XM_054358146 | 166 | F: CCAGTTGCCTTCTCCCTGG |
| R: CTGAGATGCCGTCGAGGATG | |||
| Human_IL-1β | NM_000576 | 125 | F: CCAAACCTCTTCGAGGCACA |
| R: AGCCATCATTTCACTGGCGA | |||
| Human_ GAPDH | NM_001357943 | 110 | F: GACACCCACTCCTCCACCTTT |
| R: ACCACCCTGTTGCTGTAGCC |
Gene annotation: ESR, estrogen receptor; PGR, progesterone receptor; ITGβ1, integrin subunit beta 1; LIF, LIF interleukin 6 family cytokin; PAEP, progestagen associated endometrial protein; MKI67, marker of proliferation Ki-67; MUC1, mucin 1, cell surface associated; HSPA1B, heat shock protein family A (Hsp70) member 1B; GADD45A, growth arrest and DNA damage inducible alpha; DDIT3, DNA, damage inducible transcript 3; DUSP1, dual specificity phosphatase 1; ATF4, activating transcription factor 4; JUNB, JunB proto-oncogene, AP-1 transcription factor subunit; JUND, JunD proto-oncogene; AP-1 transcription factor subunit; MAPK, mitogen-activated protein kinase; IL8, interleukin-8; ZO-1, tight junction protein 1; TNF-α, tumor necrosis factor; IL-6, interleukin-6; IL-1β, interleukin-1β; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.