Figure 4.

Spatial analysis of intra-amyloid neutrophil recruitment. Immunohistochemistry was used to specifically analyze neutrophils in tissues 8 days post the implantation of either ALλ(CLA) (A) or ALκ(TAL) (B) amyloidomas. H&E staining was performed to identify polymorphonuclear cells and other immune infiltrate. Ly6G staining was used to identify intact neutrophils and neutrophil membrane remnants. Citrullinated histone 3 staining was used to identify neutrophil extracellular traps. Data is representative of two experimental replicates with 5 animals per group. Scale bars = 50μm.