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. 2024 Nov 12;15:1487250. doi: 10.3389/fimmu.2024.1487250

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Copyright © 2024 Hancock, Vlasyuk, Foster, Macy, Wooliver, Balachandran, Williams, Martin, Kennel, Heidel, Wall and Jackson

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the neutrophil-amyloid relationship. Flow cytometry analysis of human neutrophil (A) or thioglycolate-elicited murine neutrophil (B) phagocytosis of pHrodoRed-labeled ALλ(CLA) extract or ALκ(TAL) extract. Primary neutrophil ROS productions was analyzed by flow cytometry in the presence of indicated amyloid material. (C). Effect of H₂O₂ treatment of amyloid extract and fibril degradation (D). Statistical significance was determined by unpaired Student’s t-test ****p<0.0001.