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. 2024 Aug 12;5(11):101047. doi: 10.1016/j.xplc.2024.101047

Figure 7.

Figure 7

Expression levels of GhSAMS1 and GhSAMS2 after H + S treatment and assessment of insect resistance.

(A) qRT-PCR detection of GhSAMS1 and GhSAMS2 in cpk33, cpk74, cpk33/74, CPK33-OE, CPK74-OE, and WT materials after H + S treatment. GhUB7 was used as the internal control. Means ± SE (n = 3).

(B) VIGS of GhSAMS1 and GhSAMS2. VIGS-GhCLA was the positive control, and VIGS-n was the negative control. The red line represents 5 cm.

(C) Expression levels of GhSAMS1 and GhSAMS2 in leaves of VIGS-GhSAMS1 and VIGS-GhSAMS2 plants.

(D) Comparison of the body size of S. litura larvae after 5 days of non-selective feeding on WT, VIGS-GhSAMS1, and VIGS-GhSAMS2 plants.

(E) Average weight changes of S. litura larvae after 5 days of continuous feeding on WT, VIGS-GhSAMS1, and VIGS-GhSAMS2 leaves. Means ± SE (n = 12). Statistical analyses were performed using Student’s t-test. ∗∗∗p < 0.001.