Table 1.

Evaluation of insect resistance in plants of the GhCPK mutant library for defense against chewing pests.

Mutant line	Mutated gene ID	Chewing pest resistance	Average editing efficiency
cpk1	Gh_A01G0621	ns	23.76%
cpk6	Gh_A02G1635	+	100.00%
cpk15	Gh_A05G1571	+	100.00%
cpk16	Gh_A05G2355	ns	99.08%
cpk18	Gh_A05G3246	ns	78.81%
cpk24	Gh_A09G1033	− − −	93.88%
cpk26	Gh_A09G1157	ns	100.00%
cpk27	Gh_A09G1248	ns	100.00%
cpk29	Gh_A10G0886	ns	100.00%
cpk33	Gh_A11G1615	ns	100.00%
cpk37	Gh_A12G2686	−	100.00%
cpk39	Gh_A13G0563	−	76.02%
cpk40	Gh_A13G0566	ns	100.00%
cpk45	Gh_D02G0183	− −	70.21%
cpk46	Gh_D02G0663	ns	99.28%
cpk47	Gh_D02G1973	ns	100.00%
cpk48	Gh_D03G0087	ns	100.00%
cpk51	Gh_D04G0895	ns	100.00%
cpk52	Gh_D04G0900	ns	99.69%
cpk53	Gh_D04G1271	ns	100.00%
cpk61	Gh_D07G1198	− − −	100.00%
cpk63	Gh_D08G0142	ns	100.00%
cpk64	Gh_D09G1054	−	100.00%
cpk67	Gh_D09G1249	ns	27.64%
cpk71	Gh_D10G2029	− −	100.00%
cpk74	Gh_D11G1774	ns	100.00%
cpk75	Gh_D11G3329	ns	100.00%
cpk76	Gh_D12G0121	ns	100.00%
cpk77	Gh_D12G2743	ns	100.00%
cpk79	Gh_D13G0560	+	99.78%
cpk80	Gh_D13G0561	ns	96.52%
cpk6/48	Gh_A02G1635 and Gh_D03G0087	+	100.00%
cpk8/47	Gh_A03G1505 and Gh_D02G1973	ns	100.00%
cpk10/52	Gh_A04G0467 and Gh_D04G0900	ns	100.00%
cpk13/54	Gh_A04G1429 and Gh_D04G1486	+++	100.00%
cpk20/60	Gh_A06G1772 and Gh_D06G2206	ns	98.45%
cpk21/61	Gh_A07G1099 and Gh_D07G1198	ns	100.00%
cpk29/69	Gh_A10G0886 and Gh_D10G0863	ns	99.54%
cpk30/70	Gh_A10G1195 and Gh_D10G1303	ns	100.00%
cpk33/74	Gh_A11G1615 and Gh_D11G1774	+++	100.00%
cpk36/76	Gh_A12G0109 and Gh_D12G0121	ns	100.00%
cpk37/77	Gh_A12G2686 and Gh_D12G2743	−	58.89%

Note: “+” indicates insect resistance, “−” indicates insect susceptibility, and “ns” indicates no difference.