FIG. 2.

Sensitivity of IE1 and IE2-specific RT-PCRs. The indicated numbers of in vitro-synthesized, polyadenylated IE1 and IE2 RNA molecules were subjected to the respective RT-PCRs (for primers and probes, see the map in Fig. 1). (Left panel) Ethidium bromide-stained gels verifying the correct sizes of the amplificates. M, 100-bp size markers. (Right panel) Corresponding Southern blot autoradiographs obtained after hybridization with the γ-³²P-end-labeled oligonucleotide probes IE1-P and IE2-P directed against the exon 3-exon 4 splicing junction of IE1 and the exon 2-exon 3 splicing junction of IE2, respectively.