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. 2024 Oct-Dec;16(4):251–259. doi: 10.18502/ajmb.v16i4.16741

Table 1.

The primers used in cytokine genes expression level assay by Real-Time RT-PCR

Gene Forward (5'-3') Reverse (5'-3')
IL-4 tgacggtcattctcttctgcctc aggagagtgtgttgaggtgctg
IL-12 acctccctagggcctcaccag ggccttgtgaagttcactgttc
IL-5 ggaagctctggcaacactattc tgcttcactctccgtcgctcc
IL-10 gccagccaaggcacgaacacc accctgccaaaggcagctcgg
TNF-α tgcactttggggtgatcggcc agccaccggcttgtcattatcg
IFN-γ atgttggctcttcagttctg catctgagttatctgcattc
IL-2 gttatgctttctcagagcaaccc gctaaatttagcacctgctccac
GAPDH gtagccatcaatgatccct aaggctgagaatgggaagct

IL: Interleukin, TNF: Tumor Necrosis Factor, IFN: Interferon.