Concentrations of auxin and MpIAA are dynamic during gemma development.
(A–C) Time-course imaging of MpIAA-mSC-I treated with mock (A), 100 μM MG132 (B), and 50 μM l-kynurenine and 50 μM yucasin (C).(D) Concentration dynamics of free IAA in germinating gemmae (n = 3, error bars: SD). Liquid chromatography–mass spectrometry quantification shows that total IAA levels drop following gemma germination.(E) Time-course imaging of MpTIR1-mSC-I shows that its accumulation levels gradually increase during gemma germination. Scale bars, 100 μm.