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. 2024 Jul 9;5(11):101039. doi: 10.1016/j.xplc.2024.101039

Figure 4.

Figure 4

MpARF protein accumulation in gemmae is post-translationally regulated by the proteasome.

(A) qRT–PCR of MpARF1 and MpARF2 transcripts shows stable gene transcription during gemma germination (n = 3, Student’s t test p < 0.05).(B–D) Time-course imaging of MpARF1, MpARF2, and MpARF3 protein fusions to mScarlet-I in germinating gemmae in the control treatment or upon treatment with 100 μM MG132.(E) Quantification of MpARF1, MpARF2, and MpARF3 protein accumulation after germination, expressed as FC relative to that in dormant gemmae (≥30 nuclei quantified per image, n = 3, error bars: SE). Scale bar, 50 μm.