Figure 6.
Reprogramming of transcriptional auxin response capacity during early gemma development.
(A) PCA plot of RNA-seq transcriptomes of dormant gemmae and gemmae grown in control medium for 3.5 and 8 h.
(B) Volcano plots show the number of significantly upregulated (blue) and downregulated (red) genes (padj < 0.05) at 3.5 and 8 h relative to the 0-h time point.(C) Venn diagram illustrates the overlap of DEGs in 3.5- and 8-h gemmae.(D) Transcriptional dynamics (TPM) of auxin-responsive genes during gemma germination.(E) Plot of log2(FC) differently regulated genes between 2- and 4.5-h-old gemmae treated with mock medium or auxin. Plot shows FC in average TPM counts of all differentially regulated genes at the 2 time points. Commonly regulated genes are marked in black; genes with opposite regulation at 2 and 4.5 h are indicated in blue and red, respectively.