Table 1.
Main components of Bushen Huoxue decotion identified by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS)
| Num | Compound | RT (min) |
Molecular weight (Da) | Formula | Ionization model | Class I | Class II |
|---|---|---|---|---|---|---|---|
| 1 | Cryptochlorogenic acid (4-O-Caffeoylquinic acid) | 2.79 | 3.54E+02 | C16H18O9 | [M-H]- | Phenolic acids | Phenolic acids |
| 2 | N-Feruloylagmatine | 2.91 | 3.06E+02 | C15H22N4O3 | [M+H]+ | Alkaloids | Phenolamine |
| 3 | 3,4-Dihydroxybenzoic acid (Protocatechuic acid) | 2.58 | 1.54E+02 | C7H6O4 | [M-H]- | Phenolic acids | Phenolic acids |
| 4 | Protocatechualdehyde | 3.12 | 1.38E+02 | C7H6O3 | [M-H]- | Others | Aldehyde compounds |
| 5 | Luteolin-4'-O-glucoside | 4.06 | 4.48E+02 | C21H20O11 | [M+H]+ | Flavonoids | Flavones |
| 6 | Brevifolin carboxylic acid | 3.05 | 2.92E+02 | C13H8O8 | [M-H]- | Phenolic acids | Phenolic acids |
| 7 | Gallic acid | 1.81 | 1.70E+02 | C7H6O5 | [M-H]- | Phenolic acids | Phenolic acids |
| 8 | Paeonilactone C | 4.34 | 3.18E+02 | C17H18O6 | [M+H]+ | Terpenoids | Monoterpenoids |
| 9 | Quercetin-5-O-β-D-glucoside | 3.82 | 4.64E+02 | C21H20O12 | [M+H]+ | Flavonoids | Flavonols |
| 10 | Quercetin-3-O-glucoside (Isoquercitrin) | 3.82 | 4.64E+02 | C21H20O12 | [M+H]+ | Flavonoids | Flavonols |
| 11 | Cistanoside A | 3.48 | 8.00E+02 | C36H48O20 | [M-H]- | Phenolic acids | Phenolic acids |
| 12 | Luteolin-7-O-glucoside (Cynaroside) | 4.09 | 4.48E+02 | C21H20O11 | [M+H]+ | Flavonoids | Flavones |
| 13 | Kaempferol-4'-O-glucoside | 4.05 | 4.48E+02 | C21H20O11 | [M+H]+ | Flavonoids | Flavonols |
| 14 | Quercetin-3-O-galactoside (Hyperin) | 3.82 | 4.64E+02 | C21H20O12 | [M+H]+ | Flavonoids | Flavonols |
| 15 | Saikosaponin A | 6.37 | 7.80E+02 | C42H68O13 | [M-H]- | Terpenoids | Triterpene Saponin |
| 16 | Isoferulic Acid | 4.03 | 1.94E+02 | C10H10O4 | [M-H]- | Phenolic acids | Phenolic acids |
| 17 | Echinacoside | 3.19 | 7.86E+02 | C35H46O20 | [M-H]- | Phenolic acids | Phenolic acids |
| 18 | Saikogenin Q | 5.54 | 4.88E+02 | C30H48O5 | [M+H]+ | Terpenoids | Triterpene |
| 19 | Calycosin-7-O-glucoside | 3.56 | 4.46E+02 | C22H22O10 | [M+H]+ | Flavonoids | Isoflavones |
| 20 | Catalpol | 1.19 | 3.62E+02 | C15H22O10 | [M-H]- | Terpenoids | Monoterpenoids |
Notes: Num.: number; Compounds: compound name; RT: retention time; Class: classification of compounds; Ionization model: M+H is positive charged, M-H is negative charged.