Figure 3. Expression of angiogenesis-associated molecules in granulation tissue of diabetic rats at days 7 and 14 post-surgery and the effect of ZYP formula.

A: Western blotting images at day 7 post surgery; B: after 7 d of surgery, statistical analysis of RAGE expression; C: after 7 d of surgery, statistical analysis of NF-κB p65 expression; D: after 7 d of surgery, statistical analysis of HIF-1α expression; D: after 7 d of surgery, statistical analysis of VEGF expression; F: Western blotting images at day 14 post surgery; G: after 14 d of surgery, statistical analysis of RAGE expression; H: after 14 d of surgery, statistical analysis of NF-κB p65 expression; I: after 14 d of surgery, statistical analysis of HIF-1α expression; J: after 7 d of surgery, statistical analysis of VEGF expression. Normal group: normal rats with skin ulcer; Model group: non-intervention group after the establishment of diabetic ulcer model; ZYP group: after modelling diabetic ulcer, treatment with ZYP formula; rhEGF group: after modelling diabetic ulcer, treatment with epidermal growth factor; SSD group: after modelling diabetic ulcer, treatment with 1% sulfadiazine silver cream. β-actin: beta-actin; RAGE: advanced glycation end products receptor; NF-κB p65: nuclear factor-κB p65; HIF-1α: hypoxia-inducible factor-1α; VEGF: vascular endothelial growth factor; ZYP: Zuyangping; rhEGF: epidermal growth factor; SSD: 1% sulfadiazine silver cream. Values are shown as mean ± standard deviation (n = 3). All the treated groups were compared with the model group using the Dunnett’s test. Student’s t-test was used to compare differences between the normal and model groups. ^aP < 0.05, compared with the normal group; ^bP < 0.05, compared with the model group.