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. 2024 Nov 25;14:29144. doi: 10.1038/s41598-024-80369-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — Differentiation and maturation of SH-SY5Y cells in 2D and 3D cultures assessed by flow cytometry analysis. (A) FACS analysis on day 6 of differentiation. The black arrow indicated the population of undifferentiated cells (B) FACS analysis of cells cultured in maturation media without RA. The black arrow indicates the population of undifferentiated (C) Comparison of neuronal marker expression in 2D cell cultures with and without RA treatment. (D) Comparison of neuronal marker expression in 3D spheroids cultured in 5% hiFBS with and without RA treatment.