Fig. 2.

Phylogenetic analysis of 2DBD-NRs relationships across metazoans. Maximum Likelihood (ML) tree (same parameters as in Fig. 1; values below branches indicate statistical support based on 1000 bootstraps) based on the ClustalW-aligned concatenated conserved domains (DBD1, DBD2, and LBD) of the two sequences found in the genome of L. stagnalis (present study, indicated by red dots), and 60 sequences identified by Wu and Loverde (2023) from various lineages (Brachiopoda, Rotifera, Platyhelminthes, Annelida, Mollusca, Nematoda, Echinodermata, Cephalochordata, indicated by the different colours) and having the three domains complete (as verified using Conserved Domains DataBase). The different NR7 groups referred to in the text are indicated on the right in blue, red and grey. Alignment available upon request. See also Supplementary Fig. SF3 for detailed clade composition).