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. 2024 Nov 25;14:29213. doi: 10.1038/s41598-024-78520-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — Representative whole mount in situ hybridisation (WMISH) expression profiles for a selection of 2DBD-NRs, NRs, GPCRs and ovipostatin differentially expressed between early veliger stage and adult reproductive organs (paired albumen and prostate glands). The expression patterns of these receptors and ovipostatin in early-veliger-stage larvae are broad, and generally similar; all genes are robustly expressed in ectodermal cells covering the foot (black arrowheads), surrounding the stomodaeum (white asterisk), the mantle (white arrowhead) and the cephalic lobes (yellow arrowheads). In adult tissues ovipostatin is highly expressed in the prostate and effectively undetectable in the albumen gland as expected. Qualitative differences are also evident for many of the receptors surveyed, and hint at possible roles in maintaining these simultaneously active reproductive organs. Representative scale bars are 100 mm.