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. 2024 Nov 25;14:29133. doi: 10.1038/s41598-024-72408-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — SLC4A4 knockdown suppresses growth, viability and colony formation of prostate cancer (PCa) cells (A, B) Western blot analysis and quantitative reverse transcription polymerase chain reaction (qRT-PCR) confirmed the knockdown of SLC4A4 in PCa cells (C) Knockdown of SLC4A4 reduced the growth and viability of PCa cells compared to those transduced with control shRNA (shCON) (D) SLC4A4 knockdown reduced colony formation of PCa cells. Cells were previously transfected with control shRNA and SLC4A4 shRNA, seeded in 10 cm Petri dish, and allowed to grow for 2 weeks. The colonies were fixed with 4% paraformaldehyde, stained with crystal violet and imaged using a gel doc. The number of colonies in each sample was counted using Image J software. Graphic representation of the number of colonies showed a significant decrease in the SLC4A4 knockdown group compared to the control (**p < 0.05). The results are presented as means ± standard error of three independent experiments.