Fig. 5.

Confirmation of the protein-coding potential of InSETG-4 using mass spectrometry (MS). A The diagram of the vector transfected into 293FT cells for LC–MS/MS analysis is shown above the representative mass spectra of the four detected tryptic peptides, with y ions indicated in blue and b ions in red. B Left, the major ORF sequence of InSETG-4 with the four peptides detected by MS underlined and marked in purple. Right, summary of the InSETG-4 peptides detected in different InSETG-4 transcripts and biological replicates. The peptides detected for the GFP expressed from an independent promoter are shown as positive controls