Fig. 2.

TPPU reversed the LPS-induced reduction in EMCN expression. a, b Representative IF image and quantification analysis of EMCN in HUVECs and cocultured HUVECs and PDLSCs. Scale bar = 100 µm. c, d Western blot and quantification analysis for EMCN in HUVECs and cocultured cells. Full-length blots/gels were presented in Additional file 3. e, f RT‒qPCR analysis showing the expression levels of EMCN and ICAM1 in HUVECs and cocultured HUVECs and PDLSCs. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001